Abnormal expression or activation of proteins plays an important role in the pathogenesis of human diseases and such proteins can be important targets for therapeutic interventions

Abnormal expression or activation of proteins plays an important role in the pathogenesis of human diseases and such proteins can be important targets for therapeutic interventions.

(a) Focusing particularly on techniques and approaches, discuss how you would carry out a mass spectrometry based proteomic investigation to identify and quantify proteins that are abnormally expressed in diseased tissues, when compared to normal tissues.

(13marks)

(b) Critically discuss the evolution of technology for development of various types of monoclonal antibody-based products that have been approved for the treatment of cancer patients. Using relevant examples, discuss the mechanisms by which such monoclonal antibody-based drugs produce their therapeutic effects.

(12 marks)