using qRT-PCR to measure mRNA for YFG

using qRT-PCR to measure mRNA for YFG, you notice that mRNA stops increasing after 60 minutes, which implies that new YFG transcripts are no longer made

after this time. You come up with 3 hypotheses for how this might work:

(1) ERa does not remain bound to the promoter after 60 min,

(2) antisense transcription from NG interferes with YFG transcription, or

(3) a histone writer protein is recruited and adds repressive marks to YFG.

In the boxes below, draw the histograms you would expect to obtain in each Of these scenarios.

3

+E2, 120 min

ChlP-seq ERa

CNP.eq

H3K27me3

ChlP•seq

H3K36me3

Hyl_xsthesis +E2, 120 min

Chip-seq ERa

H3K27me3

seq

H3K36me3

2 +E2. 120 min

IP.seq ERO

H3K2,’me3

H3K36me3

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